Measurement of Noncovalent Complexes with High m/z by Electrospray Time-of-Flight Mass Spectrometry
Identifieur interne : 003B47 ( Main/Exploration ); précédent : 003B46; suivant : 003B48Measurement of Noncovalent Complexes with High m/z by Electrospray Time-of-Flight Mass Spectrometry
Auteurs : I. V. Chernushevich [Canada] ; W. Ens [Canada] ; K. G. Standing [Canada]Source :
- NATO ASI Series [ 1389-2185 ]
Abstract
Abstract: In the last few years the electrospray (ES) ionization technique has proved to be gentle enough to obtain information on the higher-order structure of biomolecules [1]. However, most ES-MS studies with proteins have been carried out in acidic solutions. Such acidic solutions, particularly in combination with organic solvents, denature biopolymers, which then acquire sufficient charge to be detected within the limited m/z range of most commercial quadrupole mass spectrometers. These conditions are usually sufficiently severe so as to destroy any higher order structure, which must be examined closer to physiological conditions. There the charge acquired may be much lower, resulting in m/z values beyond the range of normal quadrupole instruments (see for example [2]).
Url:
DOI: 10.1007/978-94-015-9046-4_8
Affiliations:
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V." last="Chernushevich">I. V. Chernushevich</name><affiliation wicri:level="4"><country xml:lang="fr">Canada</country><wicri:regionArea>Physics Department, University of Manitoba, R3T 2N2, Winnipeg, Manitoba</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Ens, W" sort="Ens, W" uniqKey="Ens W" first="W." last="Ens">W. Ens</name><affiliation wicri:level="4"><country xml:lang="fr">Canada</country><wicri:regionArea>Physics Department, University of Manitoba, R3T 2N2, Winnipeg, Manitoba</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Standing, K G" sort="Standing, K G" uniqKey="Standing K" first="K. G." last="Standing">K. G. Standing</name><affiliation wicri:level="4"><country xml:lang="fr">Canada</country><wicri:regionArea>Physics Department, University of Manitoba, R3T 2N2, Winnipeg, Manitoba</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="s" type="main" xml:lang="en">NATO ASI Series</title><idno type="ISSN">1389-2185</idno><idno type="ISSN">1389-2185</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1389-2185</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: In the last few years the electrospray (ES) ionization technique has proved to be gentle enough to obtain information on the higher-order structure of biomolecules [1]. However, most ES-MS studies with proteins have been carried out in acidic solutions. Such acidic solutions, particularly in combination with organic solvents, denature biopolymers, which then acquire sufficient charge to be detected within the limited m/z range of most commercial quadrupole mass spectrometers. These conditions are usually sufficiently severe so as to destroy any higher order structure, which must be examined closer to physiological conditions. There the charge acquired may be much lower, resulting in m/z values beyond the range of normal quadrupole instruments (see for example [2]).</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Manitoba</li></region><settlement><li>Winnipeg</li></settlement><orgName><li>Université du Manitoba</li></orgName></list><tree><country name="Canada"><region name="Manitoba"><name sortKey="Chernushevich, I V" sort="Chernushevich, I V" uniqKey="Chernushevich I" first="I. V." last="Chernushevich">I. V. Chernushevich</name></region><name sortKey="Ens, W" sort="Ens, W" uniqKey="Ens W" first="W." last="Ens">W. Ens</name><name sortKey="Standing, K G" sort="Standing, K G" uniqKey="Standing K" first="K. G." last="Standing">K. G. Standing</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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